Hematological properties of mutant lines MRI26194 and MRI53426. Representative Giemsa-stained smears (A) and standard error of the mean (SEM) images (B) of erythrocytes from wild-type (WT; +/+) and both mutant lines. Hematological properties from an automated blood count. (C) Mean cell volume (MCV), mean cell hemoglobin (MCH), RBC (erythrocyte) count, and hemoglobin (HGB) by volume (n = 14-36 per group). (D) Mean values for volume, surface area, ratio of surface area to volume, and distance from channel exit of cell populations for WT (3 mice, 642 cells) and MRI2694 (2 mice, 233 cells) erythrocytes. (E) Osmotic fragility of erythrocytes (n = 8-35 per group). (F) Erythrocyte lifespan (n = 4 per group); dashed line is 50%. (G) Erythrocyte deformability as measured by ektacytometry (n = 3-6 per group). (H) Retention of erythrocytes in an in vitro spleen bead filtration model (n = 7 per group). Mean and SEM are indicated, except for microfluidic results, where standard deviation is indicated. Mann-Whitney U test was used to calculate P values. *P < .05, **P < .01, ***P < .001 between MRI26194 and WT. #P < .05, ##P < .01, ###P < .001 between MRI53426 and WT. NS, not significant.