Sptb-mutant erythrocytes are more susceptible to retention in an in vitro filtration model and macrophage phagocytosis. Relative retention rates of uninfected (Ai) or infected (Aii) mutant cells compared with uninfected WT cells in an in vitro spleen retention bead assay. Phagocytized cells quantified by fluorescent label (atto633) and macrophages by bright field (Bi-ii). Mann-Whitney U test was used to calculate P value. *P < .05, **P < .001.