Phenotypic analysis of OMs and BM-derived macrophages from neonatal and adult donors. Flow cytometric analysis (representative data) of freshly isolated NCCs, neonatal BM (NBM) cells, digested (after flushing BM) long bones (8-week digested bone) and flushed BM cells (8-week BM) from 8-week-old mice. An analysis of digested bones and BM from 2 age groups was done to ensure that the identified phenotypes were not confined to a single developmental stage. Dot plots displaying similarly gated events (top to bottom) containing phenotypically similar cells are shown. Fluorescence minus one (FMO) controls collected for these assays are shown in supplemental Figure 1. Additional phenotypic data from these analyses are shown in supplemental Figure 3. Please note that BM-derived macrophages express M-CSFR as expected. However, 2-day– and 8-week–derived BM macrophages do not contain the subgroup of CD166⁺M-CSFR⁺ cells.