C/EBPα induction and CBF fusion protein knockdown induce similar changes in the transcriptional program. (A) Hierarchical clustering of RNA-seq data by log2 fold FPKM (fragments per kilobase of transcripts per million mapped reads) values of genes differentially expressed (twofold change) after C/EBPα induction by E2 or vehicle as indicated (4 days for Kasumi-1 and 2 days for SKH-1). (B) Comparison of up- and downregulated genes after C/EBPα-ER induction in Kasumi-1 and SKH1 cells with genes changing expression after RUNX1-ETO and RUNX1-EVI1 knockdown (KD) or control siRNA treatment (MM). (C) Number of genes changing expression at least 1.5-fold after C/EBPα induction in Kasumi-1 and SKH1 cells as compared with RUNX-EVI1 or RUNX1-ETO knockdown. The P values in panel C were calculated with Fisher’s exact test using the total set of genes expressed in each condition as the background.
