Glycolysis-associated genes and H3K9 histone demethylases are upregulated under hypoxia in MM. (A) Gene ontology analysis of hypoxia-inducible genes (normoxia vs hypoxia expression, fold change >2.0) of 5 cell lines (RPMI-8226, KMS-11, KMS-12-BM, MM.1S, and U266) and samples from patients MM (n = 4) cultured in normoxia or chronic hypoxia (1% O₂) for 48 hours. (B) Commonly upregulated genes (fold change >2.0) in both cell lines and primary samples. (Upper) Diagram showing number of upregulated genes. (Lower) Gene symbols of upregulated genes. Red genes: H3K9 demethylases. (C) Quantitative gene expression change of H3K9 methylases and demethylases under chronic hypoxia. Y-axis: log₂-scale FC (fold change). Pt, primary sample.