Engraftment of AA iPSC WT, B*40:02^mut, and 6pLOH⁺HSCs (CD34⁺iPSC HSPCs) in immunodeficient mice. (A) Human CD34⁺ and CD45⁺ cells in different organs of young BRGS mice at 10 to 12 weeks after transplantation of WT, B*40:02^mut and 6pLOH⁺ CD34⁺ iPSCs. (Left panel) The percentages of human hematopoietic cells represent the mean ± SEM of 3 WT, 2 B*40:02^mut, and 3 6pLOH⁺ cell recipients. (Right panel) A representative set of FCM results is shown. The numbers below the gated area indicate the mean ± SEM of the positive cell percentages. (B) Human myeloid cells in the BM of a young mouse transplanted with 6pLOH⁺ iCD34⁺ cells. Representative results of (upper panel) hematoxylin and eosin staining and (lower panel) human myeloperoxidase staining are shown. (C) Engraftment of myeloid and lymphoid lineage cells in the different organs. The percentages of each cell lineage in (top left panel) the BM, (top right panel) spleen, and (bottom panel) PB are shown. The data represent the mean ± SEM of the percentages of each marker-positive cell obtained from 3 WT, 2 B*40:02^mut, and 3 6pLOH⁺ cell recipient mice. (D) HLA expression profiles of human B cells isolated from the spleen of the transplanted mice with the indicated iCD34⁺, (upper panels) HLA-A2402, and (lower panels) HLA-B4002 expression by CD19⁺ lymphoid cells in the spleens. (E) Morphology of CFU-GEMM–derived colonies from the BM iCD34⁺ cells. (Left) CFU-GEMM–derived colonies and (right) the constituent cells prepared by the cytospin are shown. FSC-A, forward scatter area; FSC-H, forward scatter height.