Dock8 deficiency cripples the transition from naïve B cells to unswitched memory B cells. Enriched B cells from PBMCs of HCs and Dock8 patients were stimulated with sAgs for varying lengths of time and fixed and stained with anti-pERK and pBtk antibody for flow cytometry. (A-B) The quantification of MFI of pERK (A) and pBtk (B) in naïve or memory B cells from HCs or Dock8 patients. (C) Flow cytometry analysis of naïve and memory B cells from HCs and Dock8 patients distinguished by IgD and CD27 staining. (D) The flow plot of CD19 expression in total B cells of HCs and Dock8 patients. (E-F) The quantification of MFI of CD19 and CD81 in naïve or memory B cells from HCs or Dock8 patients with flow cytometry (patients 1-3). Shown are the mean values (±SD) from 3 independent experiments. *P < .01; **P < .001.