Impact of maintenance therapy on the prognostic value of tumor burden biomarkers at diagnosis. Kaplan-Meier estimates of PFS in patients with maintenance treatment based on TMTV (A), CTC (B), cfDNA (C), and TMTV and cfDNA combined (D). Of the 16 patients with combined high or low levels, 13 had high cfDNA levels and a low TMTV, and 3 had low cfDNA levels and a high TMTV.