Coculture with M2-MΦs promotes expansion of hUCB CD34⁺cells and 6-week CAFCs ex vivo. (A) Diagram illustrating the experimental design for ex vivo hUCB CD34⁺ cell expansion and analyses. (B) Relative gene expression in M1-MΦs and M2-MΦs compared with MΦs analyzed by quantitative polymerase chain reaction. ^aP < .05 vs MΦ, ^bP < .05 vs MΦ and M1-MΦ. (C) Numbers of total cells, CD34⁺ cells, and 6-week CAFCs in the input cells and the progeny of hUCB CD34⁺ cells cultured with MΦs, M1-MΦs, or M2-MΦs or without MΦs (W/O MΦs). Data are mean ± SEM (n = 7 different units of hUCB CD34⁺ cells per group for total cell and CD34⁺ cell analyses, and n = 4 different units of hUCB CD34⁺ cells per group for CAFC assay). ^aP < .05 vs Input, ^bP < .05 vs Input and W/O MΦ, ^cP < .05 vs Input and MΦ, ^dP < .05 vs all other groups, ^eP < .05 vs all other groups except MΦ, 1-way ANOVA.