Endo H sensitivity of intracellular FV and AAT in WT, Lman1^gt1/gt1, Mcfd2^−/−, and DKO mice. (A) Liver lysates of male WT, Mcfd2^−/−, and DKO mice were treated or not with endo H and analyzed by immunoblotting with anti-FV, anti-AAT, and anti–β-actin antibodies. (B) Immunofluorescence staining of frozen sections of WT, Lman1^gt1/gt1, Mcfd2^−/−, and DKO liver for AAT. Identical staining protocols were performed simultaneously for all samples. Scale bar, 25 μm.