Genome-edited cells with t(9;11) chromosomal translocations induce AML. (A) Experimental scheme for induction of AML by genome-edited cells following transplantation in sublethally irradiated NSG recipient mice after 98 days of in vitro culture. (B) Disease-free survival of transplanted mice; *P < .009 (control vs sample 1, 1st), **P < .07 (control vs sample 2, 1st), ***P < .0001 (control vs sample 1, 2nd), and ****P < .0001 (control vs sample 2, 2nd) by log-rank test. (C-D) May-Grünwald-Giemsa staining shows blast cells in peripheral blood (PB) smear (C) and monomorphic bone marrow (BM) cells (D). Scale bar, 20 μm. (E) Increased spleen size (top) and weight (bottom) of leukemic mice compared with control; *P < .01. (F) Hematoxylin and eosin–stained sections of bone marrow, spleen, and liver. Scale bar, 100 μm.