Figure 4.
![Figure 4. Red pulp macrophages exhibit an expression pattern distinct from monocytes and monocyte-derived macrophages. Flow cytometry stainings on nonautofluorescent CD163intCD14high spleen monocytes, CD14high blood monocytes gated on FSC/SSC patterns, autofluorescent CD163highCD14low red pulp macrophages from spleen, and M-CSF and GM-CSF cultured monocyte-derived macrophages, (A) CD11a, (B) CD11b, (C) CD11c, (D) CD18, (E) CD89, (F) CD200R, (G) HLA-DR, (H) gp91PHOX (reduced NAD phosphate [NADPH] oxidase complex), (I) TLR4, and (J) IgG (polyclonal goat anti-human IgG). Mean ± SEM of n ≥ 8 are shown for each group.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/8/10.1182_bloodadvances.2017015008/3/advances015008f4.jpeg?Expires=1735479049&Signature=Qc4~r3RaIKZWSicJFO87630RxmOXqRdUwUH7BJU06ilALG5ui7~bmcBOGjDogLGXdtRklGQjiImjVRJ-WeI1Ji6naqOAQgVQnELS7VWwQV-WY3EO33LerkYJEs3-zUB9oWnpY~H55k9N~bHl8P5XwnAV6nw9p9yqtqXsQXWSjrFGAJ1mF2KTnzZk9PnynrdVZV9j5wFxN4SMRDG47EUY~CDR6VKXK5DdWgQRoMAcqiecQGjK1QP45XKjdJ2sJGGAuLmrOf5ndUfCCWxYhXU6r-SUZVEHtAKAK6KgHVlS5FbQlc-NCc-KEHgmv-mZmh53uRL4fd0fICFp-85SqERNmQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Red pulp macrophages exhibit an expression pattern distinct from monocytes and monocyte-derived macrophages. Flow cytometry stainings on nonautofluorescent CD163intCD14high spleen monocytes, CD14high blood monocytes gated on FSC/SSC patterns, autofluorescent CD163highCD14low red pulp macrophages from spleen, and M-CSF and GM-CSF cultured monocyte-derived macrophages, (A) CD11a, (B) CD11b, (C) CD11c, (D) CD18, (E) CD89, (F) CD200R, (G) HLA-DR, (H) gp91PHOX (reduced NAD phosphate [NADPH] oxidase complex), (I) TLR4, and (J) IgG (polyclonal goat anti-human IgG). Mean ± SEM of n ≥ 8 are shown for each group.
