Clonal diversity as determined by RIS analysis in nonmobilized and G-CSF/AMD3100-mobilized dogs before FV vector injection. (A) Clonal diversity in nonmobilized canines R2258 (left) and R2260 (right) at the indicated time points posttreatment. (B) Clonal diversity in mobilized canines H864 (left) and H867 (right) at the indicated time points posttreatment with G-CSF/AMD3100 and vector PGK.γC.FV. In all graphs, unique RISs are plotted based on the number of times the RIS was sequenced and normalized to the percentage of total RISs captured at each time point for each animal. Total number of unique RISs is shown on top of each bar. Captured RISs appearing at a frequency >1% in each sample are represented by boxes in each graph. Boxes are colored in white if they were identified at a single time point or in matching colors if they were identified in >1 time point at a frequency >1%. The gray portion of the graph depicts all RISs with a frequency <1% at each time point. Legends describing the precise chromosomal location of RISs are given in supplemental Figure 4.