The clonal architecture of HLA-A3101⁻granulocytes in case 2. (A) Changes in the VAF of the DNMT3A mutation during the last 6-year study (7-12 years after the diagnosis). (B) The composition of HLA-A3101⁻ granulocytes, as revealed by HLA-A allelic sequencing. 6pLOH⁻A*31:01^mut− cells accounted for 64% of the total HLA-A3101⁻ granulocytes. (C) The chronological changes in the GPI-AP⁻ granulocytes and HLA-A3101⁻ granulocytes. (D) The genotypes of 4 colonies derived from PB non-T–nonadherent cells. Whether individual colonies are positive or negative for the amplified product of A*31:01 and mutated DNMT3A (top panel) and their summary (bottom panel) are shown. (E) The results of HUMARA for HLA-A3101⁻ granulocytes. The S score was as low as 1.17, and was in line with the results of HLA-A–allelic sequencing, which showed the presence of >2 HLA-A3101⁻ HSPC clones. NSAA, nonsevere aplastic anemia.