MAP3K expression profiles in human and mouse platelets. Relative mRNA levels of MAP3Ks in human platelets (H-plt) (n = 3) (A) and in mouse platelets (M-plt) (n = 3) (B). Data are mean ± SD. (C) Expression of MEKK3, MEKK5, MLK3, A-Raf, B-Raf, and C-Raf in H-plt and M-plt was detected by western blot. Proteins extracted from HeLa cells were used as positive controls for human MAP3K detection. NIH 3T3 cells were used as positive controls for mouse MLK3, A-Raf, and C-Raf detection; mouse heart tissue was used as positive controls for MEKK3 and MEKK5 detection; and mouse leukocytes were used as positive controls for B-Raf detection. GAPDH was used to verify equal loading.