Platelets from IL4R-IbαTg mice are unaffected by the presence of VWF/p.V1316M. (A) Platelet counts in IL4R-IbαTg mice expressing only VWF/WT (100%, n = 4, red triangles) and mice overexpressing VWF/p.V1316M (n = 4, blue triangles) 4 days after hydrodynamic gene transfer. The left axis represents relative counts, and the right axis shows absolute platelet counts. (B) Platelet size in IL4R-IbαTg mice (100%, n = 4, red triangles) and IL4R-IbαTg-VWF/p.V1316M mice (n = 4, blue triangles). (C) Single platelets in blood smears from IL4R-IbαTg-VWF/p.V1316M mice. (D) Platelet counts and platelet size in Stim1^fl/flPF4-Cre⁺ (red circles), Caldaggef1^−/−P2Y12^−/− (blue squares), and talin1^fl/flPF4-Cre⁺ (green triangles) mice in the presence of VWF/WT (100%, open symbols) and VWF/p.V1316M (filled symbols). n = 3-7 mice per group. (E) Platelet aggregates found in blood smears of Stim1^fl/flPF4-Cre⁺, Caldaggef1^−/−P2Y12^−/−, and talin1^fl/flPF4-Cre⁺ mice expressing VWF/p.V1316M. Images in panels C and E were acquired as described in Figure 1.