Figure 4.
![Figure 4. VWF/p.V1316M affects platelet granule release. (A) αIIbβ3 integrin activation measured with JON/A-PE, added 10 minutes after stimulation with ADP (10 μM) and U46619 (5 μM). MFI in stimulated WT platelets (red bars) set as 100%. Red bars, VWD2B(p.V1316M) platelets. (B) Quantification of secreted [3H]serotonin from δ-granules of washed WT and VWD2B(p.V1316M) platelets (red and blue bars, respectively), stimulated or not with PAR4p (1 mM). (C) P-selectin surface expression on PAR4p-stimulated WT and VWD2B(p.V1316M) platelets (red and blue bars, respectively). Data are shown as mean ± SD of 3-17 mice per group. **P ≤ .01; ***P ≤ .001.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/12/10.1182_bloodadvances.2017014290/3/advances014290f4.jpeg?Expires=1735848368&Signature=0lWDeDmOUdnk47ETg-Fv8t9AKhOUUBX3510pj3EP-oaXvT9lGo5lz1NKajToE4MkH52FBkyGZFnwLC9A1BIjW5BgeMT~IhvVjfYZVio4qPdWDC~~ZQ9l2FJEIxRUX-lMjUyK2kpue1cXsoAJDayGOycaXuTnsbIxCGpnHdQxsQbXixv3welNThCUMU1NrC~02edh5mip1SamHl5thOR5eYKtHaIS2-FC7Ip6Ixoo4dlbfQquOcuGEtzo0kPRwke9JngJdrdMQ~cf8h1Mwd~kefFt0MQvwW1WrIYfZEGJkxxtiVs2GXKMZzUg4gXaj1jKkPE4A2cUShaDa8NDIxnBNQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
VWF/p.V1316M affects platelet granule release. (A) αIIbβ3 integrin activation measured with JON/A-PE, added 10 minutes after stimulation with ADP (10 μM) and U46619 (5 μM). MFI in stimulated WT platelets (red bars) set as 100%. Red bars, VWD2B(p.V1316M) platelets. (B) Quantification of secreted [3H]serotonin from δ-granules of washed WT and VWD2B(p.V1316M) platelets (red and blue bars, respectively), stimulated or not with PAR4p (1 mM). (C) P-selectin surface expression on PAR4p-stimulated WT and VWD2B(p.V1316M) platelets (red and blue bars, respectively). Data are shown as mean ± SD of 3-17 mice per group. **P ≤ .01; ***P ≤ .001.
