161533 TriKE does not increase TIGIT expression in HD or MDS NK cells. (A-B) Cryopreserved PBMCs from HDs (n = 6) and MDS patients (n = 5) were activated overnight with IL-15 or 161533 TriKE in the absence (A) or presence (B) of HL60 target cells and analyzed for NK cell TIGIT expression. One representative histogram (from HD) and pooled data are shown, and aggregate data are shown as mean ± standard error of the mean (SEM). Statistical analysis was performed using an unpaired Student t test.