Figure 4.
![Figure 4. Crosslinked CD16 enhances the IL-15–induced phosphor-STAT5 signal. Isolated NK cells (n = 9; by negative depletion) from HDs were stimulated with IL-15 (equal molar concentration to the IL-15 in TriKE [50 nM], BiKE [50 nM], BiKE + IL-15 [both 50 nM], or TriKE [50 nM]) with or without HL60 targets for 25 minutes before fixation and STAT5 phosphorylation analysis. Symbols represent distinct individuals, and statistical analysis was performed using a paired Student t test.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/12/10.1182_bloodadvances.2017012369/3/advances012369f4.jpeg?Expires=1735850935&Signature=XhBh-6fNBKs3rvs5OPjmMbp790NJAfvHYGLptyOBtswyGu7DFZCfYPXiJ6rmMu2Sh0tRAiAsbJf0k22unO6AABazUvYjZxb69nRA-7--tTj6ICq~9qje8R-0fRtpdS5xfOEfFgFxBubvpd00mLXKk7rTKQ0jwRfI7n3jYRAp7BT8TIxj73dE-8a~EpsMcqAQwkyKdXWa-Trr7XdaSeZ7X4a3yhNkIyO1TIO1wKkcF2Wd-YIptFvCm-ANmKA047Lv0PKsd-maSdwsr9TJaLzNJLGJYI2wrxbRSFnyHmYheWqUgn2iEuqWLf7678dxUeTud3TSduyyVAK6Xat3stOnPg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Crosslinked CD16 enhances the IL-15–induced phosphor-STAT5 signal. Isolated NK cells (n = 9; by negative depletion) from HDs were stimulated with IL-15 (equal molar concentration to the IL-15 in TriKE [50 nM], BiKE [50 nM], BiKE + IL-15 [both 50 nM], or TriKE [50 nM]) with or without HL60 targets for 25 minutes before fixation and STAT5 phosphorylation analysis. Symbols represent distinct individuals, and statistical analysis was performed using a paired Student t test.
