Improved MDS-NK cell cytotoxicity following treatment with 161533 TriKE. Purified HD-NK cells were cultured with CellTrace dye–labeled MDS BM targets (E:T ratio 2:1) for 6 hours in the presence of IL-15 (30 nM), 1633 BiKE (30 nM), 161533 TriKE (30 nM), or no treatment. MDS blast killing was assessed by gating on an intermediate CD45 and SSC low population, further gated to CD117⁺ and CD34⁺ cells within the CellTrace-positive population, and assessed for the proportion of dead cells using Live/Dead dye. Gating strategy and pooled data (mean ± SEM) are shown, and statistical analyses were performed using 1-way ANOVA.