CLL1 expression in normal healthy tissues, AML, and LSC. (A) RNA levels of CLL1 in various tumor and normal tissues based on data from TCGA and GTEx. Red dots represent cancer samples, green dots are cancer-matched normal samples, and blue dots are normal samples from GTEx. (B) TaqMan analysis of CLL1 RNA levels from isolated LSC (blue circle) and blast population (green circle) of AML patient samples, from HSC (blue circle) and multipotent progenitor cells (green circle) of healthy bone marrow samples, and from various healthy organ tissues including brain, colon, heart, kidney, liver, lung, pancreas, skin, and stomach (red open circle). Lines connecting the 2 circles indicate samples isolated from the same patient. (C) FACS analysis of CLL1 and CD33 expression in various hematopoietic lineages from healthy donor and from AML patient samples. Relative MFI is determined by dividing the MFI of the CLL1 or CD33 antibody signal by the MFI of IgG control antibody. (D) FACS analysis of CLL1 and CD33 expression in LSC of 31 AML samples. Percent-positive cells and relative MFI was determined relative to IgG control staining of CD34⁺CD38⁻ population in AML patient samples. (E) Estimated receptor copy number of CLL1 vs CD33 on AML patient samples. ACC, adrenocortical cancer; BLCA, bladder cancer; BRCA, breast cancer; CESC, cervical cancer; CHOL, cholangiocarcinoma; CMP, common myeloid progenitor; COAD, colorectal cancer; DLBC, diffused large B-cell lymphoma; GBM, glioblastoma; GMP, granulocyte-macrophage progenitor; HNSC, head and neck carcinoma; KICH/KIRC/KIRP, kidney cancers; LAML, acute myeloid leukemia (AML); LGG, brain glioma; LIHC, liver cancer; LUAD/LUSC, lung cancer; MEP, megakaryocyte-erythroid progenitor; MPP, multipotent progenitor; OV, ovarian cancer; PAAD, pancreatic cancer; PCPG, pheochromocytoma; PRAD, prostate cancer; READ, rectum cancer; SARC, sarcoma; SKCM, melanoma; TGCT, testicular tumor; THCA, thyroid cancer; UCEC, uterine/endometrial cancer; UCS, uterine cancer.