EMCN is highly expressed by HSCs in human adult BM. (A) Microarray GEPs of EMCN in highly purified human hematopoietic BM cell populations including immunophenotypic HSCs (n = 5), MPPs (n = 3), CMPs (n = 7), GMPs (n = 9), MEPs (n = 6), mature neutrophils (polymorphonuclear neutrophils [PMN]; n = 3), and monocytes (Mono; n = 4). One-way analysis of variance (ANOVA) of EMCN expression in HSCs vs MPPs, CMPs, GMPs, MEPs, PMNs, and Monos: P ≤ .0001. (B) Representative gating strategy of viable (7AAD⁻) human BM populations including all lineage-committed HPCs (L⁻34⁺38⁺; black), HSCs (L⁻34⁺38⁻45RA⁻90⁺; green), MPPs (L⁻34⁺38⁻45RA⁻90⁻; red), and LMPPs (L⁻34⁺38⁻45RA⁺90⁻; blue). (C) Average frequencies (± standard deviation; n = 7) of EMCN⁺ cells among HSCs (65.1% ± 6.8%), MPPs (26.8% ± 9.5%), LMPPs (11.1% ± 8.1%), HPCs (0.7% ± 0.5%). One-way ANOVA of EMCN⁺ cells in HSCs vs MPPs, LMPPs, and HPCs: P ≤ .0001.