Comparison of the effects of IAIPs and bikunin on neutrophil shapes. The neutrophils were incubated with HBSS, HSA (1 μM), BSA (1 μM), IAIPs (25-200 μg/mL), or bikunin (25-200 μg/mL) for 1 hour and the neutrophil shapes were observed under a fluorescence microscope. (A) The rounding and size of neutrophils were analyzed by the In Cell Analyzer 2000. Cell area and form factor were determined on 5 × 10⁴ cells on each group. Bikunin did not induce spherical shape even at high concentrations in neutrophils. The results shown are the means ± SEM of 7 experiments. (B) Typical picture from each group is shown (calcein stain; scale bars, 20 μm). NS, not significant compared with HBSS group.