Increased global translation is a characteristic of CD40L/IL-4 stimulation. (A) CLL cells were cultured either on tissue culture plastic (PL), untransfected mouse fibroblasts (NTL) or mouse fibroblasts expressing CD40L and with IL-4 in the medium (CD40L/IL-4). Histogram showing fold-increases in ATP luminescence comparing CD40L/IL-4 with NTL. The mean increase is 1.4-fold (SEM = 0.12) (n = 37). (B) Association between fold increase in ATP luminescence (comparing CD40L/IL-4 and PL conditions) and incorporation of OPP (a measure of new protein synthesis; n = 11) (R² = 0.5, P = .015). (C) Western blots showing the effects of CD40L/IL-4 culture on expression of eIF4E, 4EBP1, and ph-4EBP1 (n = 11). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is the loading control. Patient identification (ID) is given above (labeled 37-47) and fold response to CD40L/IL-4 stimulation below the autorads. (D) Correlation of eIF-4E levels without stimulation (solid circles) or following CD40L/IL-4 stimulation (open circles) with ATP response (without stimulation R² = 0.689, P = .0016 and following stimulation R² = 0.475, P = .018). (E) Correlation of phosphorylated-4EBP1 levels without stimulation (solid circles) or following CD40L/IL-4 stimulation (open circles) with ATP response (without stimulation R² = 0.492, P = .023 and following stimulation R² = 0.409, P = .046).