American Society of Hematology

Figure 5.

$Figure 5. Globin expression in day-29 hiPSC-derived erythroid cells. (A) Coexpression of globin genes (HBE, HBG1, HBG2, HBB) in individual cells visualized as the percentage of transcripts from the individual globin type vs total β-globin gene cluster transcripts (HBE + HBG1 + HBG2 + HBB). Average percentages per globin type are shown on the right for the GFP− and GFP+ fractions. A 2-tailed t test was performed to determine significant differences between GFP− and GFP+ fractions. (B) Violin plots showing normalized expression of globin genes in the GFP−– (gray) vs GFP+–sorted (green) fraction of day-29 hiPSC-derived erythroid cells. *FDR ≤ 0.05, **FDR ≤ 0.005, ***FDR ≤ 0.0005, ****P ≤ .001.$

Globin expression in day-29 hiPSC-derived erythroid cells. (A) Coexpression of globin genes (HBE, HBG1, HBG2, HBB) in individual cells visualized as the percentage of transcripts from the individual globin type vs total β-globin gene cluster transcripts (HBE + HBG1 + HBG2 + HBB). Average percentages per globin type are shown on the right for the GFP⁻ and GFP⁺ fractions. A 2-tailed t test was performed to determine significant differences between GFP⁻ and GFP⁺ fractions. (B) Violin plots showing normalized expression of globin genes in the GFP⁻– (gray) vs GFP⁺–sorted (green) fraction of day-29 hiPSC-derived erythroid cells. *FDR ≤ 0.05, **FDR ≤ 0.005, ***FDR ≤ 0.0005, ****P ≤ .001.

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