Representative laser scanning confocal microscopy images obtained after applying a 2-phase combined FISH technique based on coordinate assembly protocol for assessment of TL within the same CP CML patient’s CD34⁺38⁻compartment. (A) In phase 1, BCR-ABL⁺ and BCR-ABL⁻ cells were identified by BCR-ABL FISH staining. (B) In phase 2, PNA-Tel Q-FISH is used to restain the samples allowing telomere quantification in the BCR-ABL⁺ and BCR-ABL⁻ cells within the same individual’s CD34⁺38⁻ compartment. Image magnification ×630. Digital zoom ×2.5. DAPI, 4′,6-diamidino-2-phenylindole.