SPF and BF mice with antibiotics-induced gut flora depletion are protected from antibody-mediated TRALI. (A) Rectal temperatures, (B) lung W/D weight ratios upon CD4⁺ T-cell depletion, and anti-MHC class I antibody (clones 34-1-2s and AF6-88.5.5.3) infusion into indicated mouse groups. Vendor A and B indicate 2 vendors for laboratory mice. For the statistical analyses, only significant comparisons of interest are shown. Statistical analysis was performed with 1-way ANOVA with a Tukey post hoc test at 60 and at 90 minutes, as depicted in the accompanying table in panel A and 1-way ANOVA with a Tukey’s post hoc test (B). Data are based on 5, 13, and 10 mice per time point for BF, SPF, and BF antibiotic mice, respectively. (B) Each dot represents 1 mouse; error bars represent SD. Figure represents combinatorial data from 3 experiments. **P < .01, ****P < .0001.