Correlating VAMP-mediated exocytosis to platelet function. VAMP levels, secretion, and function are graphically correlated using our data (closed circles) and that from Koseoglu et al15 (open circles). (A) Calculated VAMP levels remaining in each of the strains tested, relative to WT, were based on the data in Figure 1B and from Graham et al.12 (B) The VAMP levels in each strain are represented by green shading. The red line indicates α-granule secretion, and the blue line indicates dense granule secretion. Secretion levels are from Figure 2 (0.05 U/mL thrombin for 2 minutes). Secretion from dense and α granules of V7−/− platelets (open symbols) were estimated based on ATP release and P-selectin exposure, respectively.15 All values are normalized to WT. Dense granule release (C) and α-granule release (D) are as in panel B and are plotted as green shading. The red line indicates tail bleeding times, and the blue line indicates the occlusive thrombosis times in the FeCl3-injury model. Data are normalized to WT. These data were taken from other figures in this article in which variations were indicated; therefore, error indicators were omitted from these graphs to improve figure clarity.
