Dissection of the immune response in the nontreated tumor. Mice bearing 2 A20 tumors were treated as in Figure 1C with IT STINGa and/or SC anti-GITR. One day posttreatment and 1 week posttreatment, mice were sacrificed and the draining lymph node of the treated site and the nontreated tumor were excised for cell population analysis. (A) Schedule of injection and organs harvest. (B-I) Cell populations in the nontreated tumor. (B) Infiltrating T cells. T-cell subsets among the T cells 1 day posttreatment (C) and 1 week posttreatment (D). Percentage of ICOS⁺ (E), CD69⁺ (F), Ki67⁺ (G) among CD8 T cells. Percentage of PD-1hi and PD1low among CD8 T cells 1 day posttreatment (H) and 1 week posttreatment (I). Represented are data pulled from 2 experiments with 3 mice per group. Error bars are SEM. Statistical significance was calculated using 1-way ANOVA. *P < .05; **P < .01; ***P < .001.