Dissection of the immune response in the draining lymph node of the treated tumor. Cell population in the draining lymph node of treated tumor harvested as described in Figure 4A. (A) Percentage of CD11b⁺ cells. (B) CD86⁺ cells among CD11b⁺ cells. T-cell subset 1 day posttreatment (C) and 1 week posttreatment (D). (E) CD8/Treg ratio. ICOS⁺ (F) CD60⁺ (G), Ki67⁺ (H), PD-1⁺ (I) positive cells among CD8 T cells. Represented are data pulled from 2 experiments with 3 mice per group. Error bars are SEM. Statistical significance was calculated using 1-way ANOVA. *P < .05; **P < .01; ***P < .001.