Co-HP almost specifically inhibited rhodocytin-induced platelet aggregation by binding to CLEC-2. (A) Inhibitory effects of Co-HP on the aggregation of washed human (i) or murine (ii) platelets (2 × 108/mL) induced by rhodocytin, collagen, or thrombin. The results from 1 experiment, representative of 3 experiments, are shown. (B) Binding between Co-HP and hCLEC-2–hFc2/integrin α2β1 was analyzed by surface plasmon resonance assay. (i) Co-HP (red line) or anti–CLEC-2 antibody (blue line) were flowed over an immobilized hCLEC2-rFc2 or a control surface. (ii) Co-HP (red line) or anti-integrin α2β1 antibody (blue line) were flowed over an immobilized recombinant integrin α2β1 or a control surface. The arrows indicate the beginning and the end of analyte perfusion. The results from 1 experiment, representative of 3 experiments, are shown. (C) Inhibitory effects of Co-HP on the aggregation of washed human platelets (1 × 109/mL) induced by hPod-CHO (1 × 106/mL). The results from 1 experiment, representative of 3 experiments, are shown.
