Co-HP inhibited hematogenous metastasis of podoplanin-expressing B16F10, but not that of podoplanin-negative LLC. (A) Study design of experimental lung metastasis. Co-HP or 1% DMSO were injected every 48 hours. B16F10-GFP cells or LLC-GFP cells were IV injected into mice through the tail vein 30 minutes after the injection of the first inhibitor. (B-C, top) Representative images of the lungs excised from Co-HP– or DMSO-injected mice 14 days after tumor injection of B16F10 (B) or LLC (C). (Bottom) Weight of the lungs excised from Co-HP– or DMSO-injected mice (n = 5 in each group) 14 days after tumor injection of B16F10 (B) or LLC (C). Mean values ± SD are shown for each group (DMSO control, ●; Co-HP, ▲). Data were analyzed using the Student t test. *P < .05. (D) Proliferation of B16F10 cells in the presence of DMSO or Co-HP. The cells were counted 24 hours after 0.3% DMSO or Co-HP administration. The cell numbers in the presence of 0.3% DMSO were set to 100%. Mean values ± SD are shown for each group (n = 6 from 3 independent experiments). Data were analyzed using the Dunnett test. n.s., not significant.