Dissociation of RGS10 from binding sites on 14-3-3γ during platelet activation. (A) Lysates were prepared from human platelets stimulated with 1 U/mL thrombin, after which 14-3-3γ was precipitated with anti-RGS10 antibody or nonimmune immunoglobulin (Ig) and probed with anti–14-3-3γ before reprobing with anti-RGS10 antibody. The bar graph summarizes results from 2 experiments. (B) Lysates were prepared from CHO cells transfected with HA-tagged RGS10 and Myc-tagged 14-3-3γ. Proteins were precipitated with an anti-HA antibody or nonimmune Ig and probed for Myc–14-3-3γ before reprobing with anti-RGS10 antibody (data are mean ± SEM, N = 3).