DCA inhibits aerobic glycolysis in convulxin-stimulated platelets. Pooled washed platelets from 5 mice were used to measure bioenergetics. OCR (A) and ECAR (B) were measured in platelets response to convulxin in the presence or absence of DCA (10 and 25 mM) with sequential injections of 1 μg/mL oligomycin, 1 μM proton ionophore FCCP, and 1 μM rotenone/0.5 μM myxothiazol. Convulxin was added at time 0 just before the microplates were put in the Seahorse extracellular flux analyzer. RP with or without DCA was used as a control. All the bioenergetic measurements were normalized to protein content per well. Values shown in bar graph are mean ± SEM, with n = 9 values per group. *P < .05 vs unstimulated platelets (no convulxin, blue bar); #P < .05 vs convulxin-stimulated platelets (violet bar). Convx, convulxin; Rot/Myx, rotenone/myxothiazol.