Coexpression of surface markers divide malignant cells into subpopulations. (A-B) Coexpression of a panel of surface markers within the malignant population of 7 patients with SS visualized by t-SNE plots colored by (A) fluorescence intensity of the indicated markers or (B) automated clustering using the PhenoGraph algorithm showing all (left) or a reduced number of clusters (right). (C) Single-cell heat maps distributed by reduced PhenoGraph cluster (left bar) and colored by fluorescence intensity of the indicated markers. Violin plots (top) display the overall expression range of the indicated markers within the total malignant population. CLA, cutaneous lymphocyte-associated antigen.