Protein expression of factors involved in thrombosis and fibrinolysis in human vein endothelial cells. HUVECs were passaged from passage 5 (P5) to 13 to induce replicative senescence and the protein expression of (A) tumor suppressor p53 (TP53), (B) heparanase (HPSE), (C) EGR1, (D) PAI-1, (E) TGFβ, and (F) PTEN was analyzed using western blot. *P < .05 vs HUVEC at P5 (set at 1); **P < .01. (G) Western blot membrane images representative for n = 6 biological replicates. GAPDH expression was examined as control for equal protein loading and is shown for the results of the same membrane. Numbers on the left indicate the molecular weight of the bands.