N-Ras^G12Dand Tet2 haploinsufficiency induce accelerated CMML-like diseases in mice. (A) Kaplan-Meier survival curve of Nras^G12D/+ (n = 33), Tet2^+/− (n = 19), Nras^G12D/+/Tet2^+/− (n = 23), and control WT littermates (n = 30). The log-rank test was used to assess statistical significance. (B) Representative flow cytometry analysis of BM cells and splenocytes (SPs) from diseased mutant mice and age-matched WT controls. The frequency of myeloid cells is determined with markers of Mac-1 and Gr-1 (Mac-1⁺Gr-1^low and Mac-1⁺Gr-1⁺). (C) Hematoxylin and eosin staining of spleen (scale bar represents 100 μm) and liver (scale bar represents 20 μm) sections from different groups of animals. Infiltration (IFT) of neoplastic cells is indicated. *P ≤ .05, **P ≤ .01, ***P ≤ .001.