Steady-state hematopoietic differentiation in 6-month-old mice. (A) Total cellularity in the BM. (B-D) Absolute numbers of HSCs, MPPs, and LSKs (B); CMPs, GMPs, and MEPs (C); and mature granulocytes and monocytes (D) in the BM. (E) Spleen (SP) weight (g) and total cellularity. (F-H) Absolute numbers of HSCs, MPPs, and LSKs (F); CMPs, GMPs, and MEPs (G); and mature granulocytes and monocytes (H) in the SP. (I) Hematoxylin and eosin staining of SP, liver, and lung sections from different groups. IFT of neoplastic cells is indicated. The absolute numbers of BM cells were calculated using an established formula that estimates that 1 femur and 1 tibiae contain 7.5% of total nucleated BM cells. Data represent mean ± standard error of the mean. One-tailed Student t tests were used to assess statistical significance. *P ≤ .05, **P ≤ .01, ***P ≤ .001.