Purified HSCs and MPPs from Nras^G12D/+/Tet2^+/−mice gain enhanced competitiveness and self-renewal. (A) Fifteen donor (CD45.2) HSCs (CD150⁺CD48⁻LSK) from Nras^G12D/+, Tet2^+/−, Nras^G12D/+/Tet2^+/−, and littermate control mice (n = 2 donors per genotype) were transplanted into lethally irradiated recipient (CD45.1) mice (n = 10 recipients per genotype) along with 3 × 10⁵ recipient BM cells. Donor-cell reconstitution in total nucleated cells and myeloid and B- and T-cell lineages was assessed in peripheral blood after transplantation. (B) Fifteen donor (CD45.2) MPPs (CD150⁻CD48⁻LSK) were transplanted into lethally irradiated recipient (CD45.1) mice (n = 10 recipients per genotype) along with 3 × 10⁵ recipient BM cells. The number of recipient mice with long-term engraftment from 2 separate rounds of transplantation is shown for each genotype. (C) Donor-cell reconstitution was assessed in peripheral blood from mice in panel B. Data represent mean ± standard error of the mean. Two-tailed Student t tests were used to assess statistical significance. *P ≤ .05, **P ≤ .01, ***P ≤ .001.