Analysis of differential gene expression in Nras^G12D/+/Tet2^+/−HSCs by RNA sequencing. (A) Clustering of differential genes by unsupervised correspondence analysis (COA). (B) List of the top-ranking upregulated and downregulated genes in Nras^G12D/+/Tet2^+/− vs WT HSCs with a fold change of >2 and false discovery rate (FDR) of <0.1 (P < .05). (C) Quantitative real-time reverse transcription polymerase chain reaction of negative regulators of JAK/STAT signaling in Nras^G12D/+/Tet2^+/− and WT HSCs from mice 2 weeks post–poly (I:C). Data represent mean ± standard error of the mean. Two-tailed Student t tests were used to assess statistical significance. *P ≤ .05, **P ≤ .01.