American Society of Hematology

Figure 2.

$Figure 2. Krt18-CreER labels HSCs. (A) Experimental setup. Krt18-CreER/YFP mice (3-6 months old) were injected with tamoxifen for 5 days and analyzed at 1, 4, 26, and ∼52 weeks after the first injection. (B) Representative flow cytometry plot at 1 week after the first tamoxifen treatment. A total of 80% to 90% of Lineage−YFP+ cells were HSCs. The numbers indicate the frequencies of cells in the indicated gate in respect to the parental gate. (C) Representative flow cytometry plots of LSK fraction separated by CD150 and CD48 to identify HSCs, MPPs, HPC1, and HPC2 at 1 week. Approximately 2% of HSCs were YFP+, whereas YFP+ cells in other immature populations were rare. (D) 30 YFP+ and YFP− HSCs were isolated and competitively transplanted. There were no significant differences in the reconstitution levels between the 2 groups (n = 3). Error bars in panel D represent SEM.$

Krt18-CreER labels HSCs. (A) Experimental setup. Krt18-CreER/YFP mice (3-6 months old) were injected with tamoxifen for 5 days and analyzed at 1, 4, 26, and ∼52 weeks after the first injection. (B) Representative flow cytometry plot at 1 week after the first tamoxifen treatment. A total of 80% to 90% of Lineage⁻YFP⁺ cells were HSCs. The numbers indicate the frequencies of cells in the indicated gate in respect to the parental gate. (C) Representative flow cytometry plots of LSK fraction separated by CD150 and CD48 to identify HSCs, MPPs, HPC1, and HPC2 at 1 week. Approximately 2% of HSCs were YFP⁺, whereas YFP⁺ cells in other immature populations were rare. (D) 30 YFP⁺ and YFP⁻ HSCs were isolated and competitively transplanted. There were no significant differences in the reconstitution levels between the 2 groups (n = 3). Error bars in panel D represent SEM.

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