Intraphagosomal bacterial containment is independent of ROS. Effect of hypoxia (0.1% O2) on bactericidal capacity of 5 × 106 neutrophils (Neu) per mL from healthy participants when cocultured with 5 × 106 CFUs per mL SA-GFP in fibrin gel. (A) Growth curves after incubating neutrophils and SA-GFP in either normoxic or hypoxic conditions and (B) corresponding TUOs (n = 6). (C) Coculture of SA-GFP (5 × 106 CFUs per mL) and increasing concentrations of neutrophils from CGD patients (n = 6) and healthy controls (n = 4) in fibrin gels. (D) TUO of SA-GFP (5 × 106 CFUs per mL) cocultured in fibrin gel with 10 × 106 neutrophils per mL of healthy control neutrophils (n = 10) vs CGD patients (n = 6). (E) Mean bacterial growth measured by GFP fluorescence after lysis of 10 × 106 neutrophils per mL from healthy controls (n = 10) and CGD patients (n = 6) by Triton X-100 16 hours after starting cocultures with 5 × 106 CFUs per mL SA-GFP in fibrin gels and (F) corresponding TUOs. All data are mean + SEM. *P < .05, as determined by Student t test for unpaired samples.
