Platelet-HMGB1 regulates neutrophil recruitment during sepsis. WT, HMGB1 Flox, and HMGB1 PF4 mice were subjected to CLP for 18 hours. (A-B) Percentage of neutrophils was assessed by flow cytometry. Numbers indicate the percentage of CD11b and Ly6G double-positive cells. (C) The total number of neutrophils in the peritoneal cavity. (D) Circulating leukocytes, (E) neutrophils, and (F) platelets were measured in WT, HMGB1 Flox, and HMGB1 PF4 mice at 18 hours after CLP. Symbols represent individual mice. Data represent mean ± SD from 3 individual experiments. Statistical difference was tested using Student t test; *P < .05.