Adoptive transfer of HMGB1 Flox-platelets improves bacterial clearance and neutrophil recruitment in HMGB1 PF4 mice after CLP. Platelets were isolated from HMGB1 Flox (Flox-plt) or HMGB1 PF4 (PF4-plt) mice. Two hundred million platelets were suspended in PBS and injected IV into HMGB1 PF4 mice 30 minutes before CLP. (A-B) peritoneal lavage fluid and blood were collected at 18 hours after CLP. Bacterial counts in (A) blood and (B) peritoneum. Statistical difference was tested using a nonparametric Mann-Whitney U statistics. (C) The total number of neutrophils in the peritoneal cavity. (D) Circulating platelets numbers at 18 hours after CLP. (E-F) Plasma cytokine levels. Blood was collected at 18 hours after CLP. Plasma (E) IL-6 and (F) HMGB1 concentrations were measured by ELISA. Symbols represent individual mice. Statistical difference was tested using Student t test; *P < .05