CMV-specific T-cell immune reconstitution and viral reactivation in HSCT patients. Longitudinal blood samples from HSCT recipients were monitored for viral reactivation and assessed for CMV-specific T-cell immunity using QuantiFERON and HLA-matched MHC-multimer analysis. (A-B) Representative MHC-multimer analyses from a NIR patient (p14) (A) and IR patient (p01) (B) are shown. Longitudinal analysis of viral load, MHC-multimer frequency, and QuantiFERON response in 2 NIR patients (p15 and p13) (A) and 2 IR patients (p01 and p15) (B) are shown. (C) Comparative analysis of the QuantiFERON IFN response and MHC-multimer frequency in IR (blue) and NIR (green) patients at 10 to 14 weeks after transplant. Linear regression lines and coefficient of determination (R²) are shown, and patients used for RNA-seq are annotated.