Figure 1
Figure 1. A C57BL/6 splenocytes were stained with NKT14 or isotype control followed by a secondary anti mouse IgG2a monoclonal and aGalCer loaded CD1d Tetramer. B C57BL/6 mice were injected with 50 µg NKT14 or isotype control. Mice were sacrifice 24 hours after dosing and splenocytes prepared, counted and stained for CD3, CD4, CD8 and aGalCer loaded CD1d Tetramer to assess specifity of iNKT cell depletion. C C57BL/6 mice were injected with 2µg aGalCer, 50 µg NKT14m or isotype control. 2 hours post dosing mice were sacrificed, splenocytes prepared and stained for CD3 and aGalCer loaded CD1d tetramers to identify iNKT cells, washed, fixed, permeabilized and stained for intracellular IFn-Gamma.

A C57BL/6 splenocytes were stained with NKT14 or isotype control followed by a secondary anti mouse IgG2a monoclonal and aGalCer loaded CD1d Tetramer. B C57BL/6 mice were injected with 50 µg NKT14 or isotype control. Mice were sacrifice 24 hours after dosing and splenocytes prepared, counted and stained for CD3, CD4, CD8 and aGalCer loaded CD1d Tetramer to assess specifity of iNKT cell depletion. C C57BL/6 mice were injected with 2µg aGalCer, 50 µg NKT14m or isotype control. 2 hours post dosing mice were sacrificed, splenocytes prepared and stained for CD3 and aGalCer loaded CD1d tetramers to identify iNKT cells, washed, fixed, permeabilized and stained for intracellular IFn-Gamma.

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