Figure 1.
Figure 1. Uptake and activation of CD8+ DCs by transfused old and IgG coated RBCs. / A) Two hours after transfusion of GFP+ fresh, stored and IgG-coated RBCs, spleens were dissociated and APC subsets analyzed using flow cytometry. Representative contour plots show the internalization of GFP+ RBCs by the CD8+ DC population. / B) CD8+ DCs with ingested GFP+ RBCs show increased expression of activation markers. Shown on the left are representative histograms depicting the shift in MHC II (top) and CD86 (bottom) expression in GFP+ (red) compared to GFP- (blue) CD8+ DCs. Shown on the right are the MHC II (top) and CD86 (bottom) median fluorescence intensity (MFI) values (mean ± SEM, n = 4). ∗∗p < 0.01.

Uptake and activation of CD8+ DCs by transfused old and IgG coated RBCs.

A) Two hours after transfusion of GFP+ fresh, stored and IgG-coated RBCs, spleens were dissociated and APC subsets analyzed using flow cytometry. Representative contour plots show the internalization of GFP+ RBCs by the CD8+ DC population.

B) CD8+ DCs with ingested GFP+ RBCs show increased expression of activation markers. Shown on the left are representative histograms depicting the shift in MHC II (top) and CD86 (bottom) expression in GFP+ (red) compared to GFP- (blue) CD8+ DCs. Shown on the right are the MHC II (top) and CD86 (bottom) median fluorescence intensity (MFI) values (mean ± SEM, n = 4). ∗∗p < 0.01.

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