Immunoblot analysis. 8% SDS-PAGE analysis of proteins eluted from aluminum hydroxide Lanes 1 and 7 are protein standard size markers. Lanes 2 and 3 are unrelated controls. Lanes 4–6 are related, unknown disease status patients without the disease haplotype. Lanes 8–10 are related, unknown affected status patients with the disease haplotype. Lanes 11–13 are affected individuals with disease haplotype. FV was detected using monolconal against the heavy chain (AHV-5146), and the Arnerrham ECF Western Blotting Detection Kit was used to develop the western blots. Mr indicates molecular range.