Figure 1
Detection of residual Ph+ cells in FACS sorted CD34+ cell subpopulations in CML patients in MMR and MR4. (A) FACS sorting of HSCs, MpPs, and MnPs. (B) Expression of BCR-ABL mRNA (on the International Scale (IS)) in the sorted CD34+ subsets. Error bars denote the median upper boundary of BCR-ABL expression (± 95% confidence interval). (C) Residual Ph+ cells as detected by iFISH in the sorted CD34+ subsets. (D) Detection of residual Ph+ cells in sorted subsets by iFISH and mRNA-based qPCR. Error bars denote SEM. (E) Combined qPCR and iFISH status of samples analyzed by both modalities.
