On-cell patch current traces of R2488Q and R2456H mutations in Xenopus oocytes. On-cell patch current traces (recorded at −applied voltage at the membrane patch (VP) = −100 mV) and current-voltage relationships from (A) uninjected oocytes and (B) oocytes previously injected with cRNA encoding WT PIEZO1, (C) PIEZO1 mutant R2488Q, and (D) PIEZO1 mutant R2456H. Single-channel slope conductances are 25 pS (wildtype), 26.5 pS (R2488Q), and 43 pS (R2456H). Supplemental Figure 3S presents a higher resolution on-cell patch trace from an oocyte expressing mutant R2456H.